Review



in fusion hd cloning kit  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TaKaRa in fusion hd cloning kit
    In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+cloning+kit/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
    Average 99 stars, based on 10117 article reviews
    in fusion hd cloning kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Construct:

    Article Title: Transmembrane domain interactions underlie NSG1 regulation of sortilin ectodomain shedding.
    Article Snippet: Primers were designed using Takara Bio’s online tool (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools) and purchased from Merck. .. Constructs were assembled using the In-Fusion Cloning Kit according to the manufacturer’s instructions (Takara Bio). .. All constructs were verified by Sanger sequencing (Eurofins Genomics LightRun service).

    Article Title: Transmembrane domain interactions underlie NSG1 regulation of sortilin ectodomain shedding
    Article Snippet: Primers were designed using Takara Bio’s online tool ( https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ) and purchased from Merck. .. Constructs were assembled using the In-Fusion Cloning Kit according to the manufacturer’s instructions (Takara Bio). .. All constructs were verified by Sanger sequencing (Eurofins Genomics LightRun service).

    Cloning:

    Article Title: Transmembrane domain interactions underlie NSG1 regulation of sortilin ectodomain shedding.
    Article Snippet: Primers were designed using Takara Bio’s online tool (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools) and purchased from Merck. .. Constructs were assembled using the In-Fusion Cloning Kit according to the manufacturer’s instructions (Takara Bio). .. All constructs were verified by Sanger sequencing (Eurofins Genomics LightRun service).

    Article Title: Transmembrane domain interactions underlie NSG1 regulation of sortilin ectodomain shedding
    Article Snippet: Primers were designed using Takara Bio’s online tool ( https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ) and purchased from Merck. .. Constructs were assembled using the In-Fusion Cloning Kit according to the manufacturer’s instructions (Takara Bio). .. All constructs were verified by Sanger sequencing (Eurofins Genomics LightRun service).

    Article Title: Rapid detection of Senecavirus A using a colloidal gold lateral flow strip
    Article Snippet: Restriction enzymes Nde I and Xho I were obtained from New England Biolabs. .. Escherichia coli Trans10, BL21 star (DE3) competent cells and Trans5K DNA marker were purchased from Beijing TransGen Biotech Co., Ltd. PrimeSTAR® HS DNA Polymerase (premix), In-Fusion Cloning Kit, and Bradford Protein Assay Kit were all purchased from TaKaRa. .. PageRuler Prestained Protein Ladder was purchased from Thermo Fisher.

    Article Title: NBR1 shuttles between the cytoplasm and nucleus and is essential for nuclear p62 body formation
    Article Snippet: Gateway LR recombination reactions were done using the Gateway cloning system (Invitrogen) and as described in the Gateway cloning technology instruction manual. .. In-Fusion cloning was done using the In-Fusion cloning kit by Takara and oligonucleotides suggested by Takara or SnapGene software ( www.snapgene.com ). .. Larger deletions were introduced with In-Fusion cloning in accordance with tabulated guidelines (Takara).

    Article Title: The identification of key salt and alkali tolerance genes in smooth bromegrass (Bromus inermis L.) by transcriptome analysis
    Article Snippet: .. The pYES2 vector was cut using HindIII-HF and BamHI enzymes from New England Biolabs, MA, USA and then combined with PCR products using the In-Fusion cloning kit from Takara, creating pYES2recombinant vectors such as pYES2-Bromus_inermis_114708, pYES2Bromus_inermis_049541, pYES2-Bromus_inermis_074327, pYES2-Bromus_inermis_295092, pYES2-Bromus_inermis_262350, pYES2-Bromus_inermis_248515, pYES2-Bromus_inermis_142424, pYES2Bromus_inermis_018614,and pYES2-Bromus_inermis_001261. ..

    Article Title: Analysing the effect of full-length and C-terminally truncated progranulin on proliferation, colony formation, and migration in HepG2 and U87 cells
    Article Snippet: .. Both inserts were further ligated in the digested vector using the In-Fusion Cloning kit by Takara, following the manufacturer’s instructions. ..

    Article Title: Analysing the effect of full-length and C-terminally truncated progranulin on proliferation, colony formation, and migration in HepG2 and U87 cells
    Article Snippet: .. Both inserts were further ligated in the digested vector using the In-Fusion Cloning kit by Takara, following the manufacturer’s instructions. ..

    Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
    Article Snippet: .. Products were separated in 2% agarose gel, and purified by Gel Out kit (EurX, Poland). pIRESneo3 plasmid (Takara Bio, USA) was cut with EcoRI (EurX, Poland), gel purified, and ESRP1 or ESRP2 cDNA were cloned with use of In Fusion cloning kit (Takara Bio, USA) according to manufacturer instruction. .. 5 μL of reaction were taken to transform attached to cloning kit chemocompetent E. Coli which seeded on LB agarose plates with 100 μg/mL of ampicillin (MilliporeSigma, USA).

    Marker:

    Article Title: Rapid detection of Senecavirus A using a colloidal gold lateral flow strip
    Article Snippet: Restriction enzymes Nde I and Xho I were obtained from New England Biolabs. .. Escherichia coli Trans10, BL21 star (DE3) competent cells and Trans5K DNA marker were purchased from Beijing TransGen Biotech Co., Ltd. PrimeSTAR® HS DNA Polymerase (premix), In-Fusion Cloning Kit, and Bradford Protein Assay Kit were all purchased from TaKaRa. .. PageRuler Prestained Protein Ladder was purchased from Thermo Fisher.

    Bradford Protein Assay:

    Article Title: Rapid detection of Senecavirus A using a colloidal gold lateral flow strip
    Article Snippet: Restriction enzymes Nde I and Xho I were obtained from New England Biolabs. .. Escherichia coli Trans10, BL21 star (DE3) competent cells and Trans5K DNA marker were purchased from Beijing TransGen Biotech Co., Ltd. PrimeSTAR® HS DNA Polymerase (premix), In-Fusion Cloning Kit, and Bradford Protein Assay Kit were all purchased from TaKaRa. .. PageRuler Prestained Protein Ladder was purchased from Thermo Fisher.

    Software:

    Article Title: NBR1 shuttles between the cytoplasm and nucleus and is essential for nuclear p62 body formation
    Article Snippet: Gateway LR recombination reactions were done using the Gateway cloning system (Invitrogen) and as described in the Gateway cloning technology instruction manual. .. In-Fusion cloning was done using the In-Fusion cloning kit by Takara and oligonucleotides suggested by Takara or SnapGene software ( www.snapgene.com ). .. Larger deletions were introduced with In-Fusion cloning in accordance with tabulated guidelines (Takara).

    Polymerase Chain Reaction:

    Article Title: The identification of key salt and alkali tolerance genes in smooth bromegrass (Bromus inermis L.) by transcriptome analysis
    Article Snippet: .. The pYES2 vector was cut using HindIII-HF and BamHI enzymes from New England Biolabs, MA, USA and then combined with PCR products using the In-Fusion cloning kit from Takara, creating pYES2recombinant vectors such as pYES2-Bromus_inermis_114708, pYES2Bromus_inermis_049541, pYES2-Bromus_inermis_074327, pYES2-Bromus_inermis_295092, pYES2-Bromus_inermis_262350, pYES2-Bromus_inermis_248515, pYES2-Bromus_inermis_142424, pYES2Bromus_inermis_018614,and pYES2-Bromus_inermis_001261. ..

    Plasmid Preparation:

    Article Title: Analysing the effect of full-length and C-terminally truncated progranulin on proliferation, colony formation, and migration in HepG2 and U87 cells
    Article Snippet: .. Both inserts were further ligated in the digested vector using the In-Fusion Cloning kit by Takara, following the manufacturer’s instructions. ..

    Article Title: Analysing the effect of full-length and C-terminally truncated progranulin on proliferation, colony formation, and migration in HepG2 and U87 cells
    Article Snippet: .. Both inserts were further ligated in the digested vector using the In-Fusion Cloning kit by Takara, following the manufacturer’s instructions. ..

    Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
    Article Snippet: .. Products were separated in 2% agarose gel, and purified by Gel Out kit (EurX, Poland). pIRESneo3 plasmid (Takara Bio, USA) was cut with EcoRI (EurX, Poland), gel purified, and ESRP1 or ESRP2 cDNA were cloned with use of In Fusion cloning kit (Takara Bio, USA) according to manufacturer instruction. .. 5 μL of reaction were taken to transform attached to cloning kit chemocompetent E. Coli which seeded on LB agarose plates with 100 μg/mL of ampicillin (MilliporeSigma, USA).

    Agarose Gel Electrophoresis:

    Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
    Article Snippet: .. Products were separated in 2% agarose gel, and purified by Gel Out kit (EurX, Poland). pIRESneo3 plasmid (Takara Bio, USA) was cut with EcoRI (EurX, Poland), gel purified, and ESRP1 or ESRP2 cDNA were cloned with use of In Fusion cloning kit (Takara Bio, USA) according to manufacturer instruction. .. 5 μL of reaction were taken to transform attached to cloning kit chemocompetent E. Coli which seeded on LB agarose plates with 100 μg/mL of ampicillin (MilliporeSigma, USA).

    Purification:

    Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
    Article Snippet: .. Products were separated in 2% agarose gel, and purified by Gel Out kit (EurX, Poland). pIRESneo3 plasmid (Takara Bio, USA) was cut with EcoRI (EurX, Poland), gel purified, and ESRP1 or ESRP2 cDNA were cloned with use of In Fusion cloning kit (Takara Bio, USA) according to manufacturer instruction. .. 5 μL of reaction were taken to transform attached to cloning kit chemocompetent E. Coli which seeded on LB agarose plates with 100 μg/mL of ampicillin (MilliporeSigma, USA).

    Clone Assay:

    Article Title: ESRP2 constrains EMT plasticity associated with ZEB1 expression in bladder cancer
    Article Snippet: .. Products were separated in 2% agarose gel, and purified by Gel Out kit (EurX, Poland). pIRESneo3 plasmid (Takara Bio, USA) was cut with EcoRI (EurX, Poland), gel purified, and ESRP1 or ESRP2 cDNA were cloned with use of In Fusion cloning kit (Takara Bio, USA) according to manufacturer instruction. .. 5 μL of reaction were taken to transform attached to cloning kit chemocompetent E. Coli which seeded on LB agarose plates with 100 μg/mL of ampicillin (MilliporeSigma, USA).



    Similar Products

    99
    TaKaRa in fusion hd cloning kit
    In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+cloning+kit/In-Fusion+HD+Cloning+Kit/pmc13123328-130-12-16
    Average 99 stars, based on 1 article reviews
    in fusion hd cloning kit - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results